Detailed information of TA system TA145960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 764117..764646
• Replicon: chromosome
• Accession: NZ_CP047321
• Organism: Staphylococcus aureus strain RJ1267

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GSF72_RS03810
• Protein ID: WP_000621175.1
• Coordinates: 764284..764646 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: GSF72_RS03805
• Protein ID: WP_000948331.1
• Coordinates: 764117..764287 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSF72_RS03775	759153..759713	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
GSF72_RS03780	759922..760401	+	480	WP_001287088.1	hypothetical protein	-
GSF72_RS03785	760394..761977	+	1584	WP_001294626.1	PH domain-containing protein	-
GSF72_RS03790	761964..762455	+	492	WP_001205910.1	PH domain-containing protein	-
GSF72_RS03795	762459..762818	+	360	WP_000581200.1	holo-ACP synthase	-
GSF72_RS03800	762884..764032	+	1149	WP_001281145.1	alanine racemase	-
GSF72_RS03805	764117..764287	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GSF72_RS03810	764284..764646	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GSF72_RS03815	764996..765997	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
GSF72_RS03820	766116..766442	+	327	WP_001052491.1	anti-sigma factor antagonist	-
GSF72_RS03825	766444..766923	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
GSF72_RS03830	766898..767668	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T145960 WP_000621175.1 NZ_CP047321:764284-764646 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T145960 NZ_CP062713:947785-948192 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT145960 WP_000948331.1 NZ_CP047321:764117-764287 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT145960 NZ_CP062713:947538-947804 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1