Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 282786..283479 Replicon plasmid unnamed1
Accession NZ_CP047312
Organism Pseudomonas putida strain YC-AE1

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag GR140_RS29310 Protein ID WP_003151133.1
Coordinates 282786..283163 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag GR140_RS29315 Protein ID WP_001172026.1
Coordinates 283144..283479 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GR140_RS29300 (GR140_29210) 278979..282008 - 3030 WP_024015041.1 Tn3 family transposase -
GR140_RS29305 (GR140_29215) 281992..282594 - 603 WP_010465829.1 recombinase family protein -
GR140_RS29310 (GR140_29220) 282786..283163 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GR140_RS29315 (GR140_29225) 283144..283479 + 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
GR140_RS29320 (GR140_29230) 283494..283828 + 335 Protein_362 transposase -
GR140_RS29325 (GR140_29235) 283852..284178 + 327 WP_000091614.1 hypothetical protein -
GR140_RS29330 (GR140_29240) 284175..284600 + 426 WP_249183623.1 hypothetical protein -
GR140_RS29335 (GR140_29245) 284524..285453 - 930 WP_003150544.1 recombinase family protein -
GR140_RS29340 (GR140_29250) 285655..285894 + 240 WP_003089107.1 ribbon-helix-helix domain-containing protein -
GR140_RS29345 (GR140_29255) 285894..286304 + 411 WP_003150546.1 putative toxin-antitoxin system toxin component, PIN family -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..504084 504084
- inside IScluster/Tn - - 276725..289301 12576


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T145944 WP_003151133.1 NZ_CP047312:282786-283163 [Pseudomonas putida]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T145944 NZ_CP062711:c4475603-4475205 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT145944 WP_001172026.1 NZ_CP047312:283144-283479 [Pseudomonas putida]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT145944 NZ_CP062711:c4475899-4475606 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References