145943

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 263818..264511 Replicon plasmid unnamed1
Accession NZ_CP047312
Organism Pseudomonas putida strain YC-AE1

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag GR140_RS29245 Protein ID WP_003151133.1
Coordinates 264134..264511 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag GR140_RS29240 Protein ID WP_001172026.1
Coordinates 263818..264153 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GR140_RS29210 (GR140_29120) 260993..261403 - 411 WP_003150546.1 putative toxin-antitoxin system toxin component, PIN family -
GR140_RS29215 (GR140_29125) 261403..261642 - 240 WP_003089107.1 ribbon-helix-helix domain-containing protein -
GR140_RS29220 (GR140_29130) 261844..262773 + 930 WP_003150544.1 recombinase family protein -
GR140_RS29225 (GR140_29135) 262697..263122 - 426 WP_249183623.1 hypothetical protein -
GR140_RS29230 (GR140_29140) 263119..263445 - 327 WP_000091614.1 hypothetical protein -
GR140_RS29235 (GR140_29145) 263469..263803 - 335 Protein_344 transposase -
GR140_RS29240 (GR140_29150) 263818..264153 - 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
GR140_RS29245 (GR140_29155) 264134..264511 - 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GR140_RS29250 (GR140_29160) 264703..265305 + 603 WP_010465829.1 recombinase family protein -
GR140_RS29255 (GR140_29165) 265289..268318 + 3030 WP_024015041.1 Tn3 family transposase -
GR140_RS32830 268403..268519 - 117 Protein_349 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..504084 504084
- inside IScluster/Tn - - 250947..270203 19256


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T145943 WP_003151133.1 NZ_CP047312:c264511-264134 [Pseudomonas putida]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T145943 NZ_CP062711:4218103-4218321 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT145943 WP_001172026.1 NZ_CP047312:c264153-263818 [Pseudomonas putida]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT145943 NZ_CP062711:4217703-4218077 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References