Detailed information of TA system TA145942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 3701824..3702707
• Replicon: chromosome
• Accession: NZ_CP047311
• Organism: Pseudomonas putida strain YC-AE1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GR140_RS16825
• Protein ID: WP_232435421.1
• Coordinates: 3701824..3702210 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: I7C3Q7
• Locus tag: GR140_RS16830
• Protein ID: WP_003250525.1
• Coordinates: 3702258..3702707 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GR140_RS16810 (GR140_16760)	3698125..3699354	-	1230	WP_070096393.1	acyl-CoA dehydrogenase	-
GR140_RS16815 (GR140_16765)	3699495..3700418	+	924	WP_004573723.1	LysR family transcriptional regulator	-
GR140_RS16820 (GR140_16770)	3700612..3701766	+	1155	WP_060537721.1	aminotransferase class V-fold PLP-dependent enzyme	-
GR140_RS16825 (GR140_16775)	3701824..3702210	-	387	WP_232435421.1	RES family NAD+ phosphorylase	Toxin
GR140_RS16830 (GR140_16780)	3702258..3702707	-	450	WP_003250525.1	DUF2384 domain-containing protein	Antitoxin
GR140_RS16835 (GR140_16785)	3702850..3703503	-	654	WP_004573726.1	oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB	-
GR140_RS16840 (GR140_16790)	3703607..3704530	+	924	WP_064614214.1	LysR family transcriptional regulator	-
GR140_RS16845 (GR140_16795)	3704579..3705409	+	831	WP_003250518.1	AraC family transcriptional regulator	-
GR140_RS16850 (GR140_16800)	3705461..3706045	+	585	WP_004573728.1	LysE family transporter	-
GR140_RS16855 (GR140_16805)	3706085..3707284	-	1200	WP_014861778.1	sugar transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-121)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14210.24 Da
• Isoelectric Point: 6.0673

>T145942 WP_232435421.1 NZ_CP047311:c3702210-3701824 [Pseudomonas putida]
MRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQPGWSAHPELTRTLGNRF
LDDGSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Nucleotide

• Length: 387 bp

>T145942 NZ_CP062711:3760411-3760797 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16956.46 Da
• Isoelectric Point: 5.7258

>AT145942 WP_003250525.1 NZ_CP047311:c3702707-3702258 [Pseudomonas putida]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL

Nucleotide

• Length: 450 bp

>AT145942 NZ_CP062711:3760787-3761116 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Antitoxin

• AlphaFold DB: I7C3Q7