Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4940959..4941575 Replicon chromosome
Accession NZ_CP047307
Organism Citrobacter freundii strain L75

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag GJV84_RS23585 Protein ID WP_003028682.1
Coordinates 4940959..4941333 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag GJV84_RS23590 Protein ID WP_043018956.1
Coordinates 4941333..4941575 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV84_RS23570 4938462..4939364 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
GJV84_RS23575 4939361..4939996 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
GJV84_RS23580 4939993..4940922 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
GJV84_RS23585 4940959..4941333 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
GJV84_RS23590 4941333..4941575 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
GJV84_RS23595 4941781..4942689 + 909 WP_003028676.1 alpha/beta hydrolase -
GJV84_RS23600 4942840..4943781 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
GJV84_RS23605 4943826..4944263 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
GJV84_RS23610 4944260..4945132 - 873 WP_003028669.1 virulence factor BrkB family protein -
GJV84_RS23615 4945126..4945725 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T145938 WP_003028682.1 NZ_CP047307:c4941333-4940959 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T145938 NZ_CP062711:3115115-3115222 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT145938 WP_043018956.1 NZ_CP047307:c4941575-4941333 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT145938 NZ_CP062711:c3115062-3115001 [Escherichia coli O157:H7]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References