Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4621608..4622361 Replicon chromosome
Accession NZ_CP047307
Organism Citrobacter freundii strain L75

Toxin (Protein)


Gene name yeeV Uniprot ID R8WN26
Locus tag GJV84_RS22170 Protein ID WP_016154457.1
Coordinates 4621984..4622361 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID -
Locus tag GJV84_RS22165 Protein ID WP_162838704.1
Coordinates 4621608..4621934 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV84_RS22105 4616666..4616932 + 267 WP_009652486.1 hypothetical protein -
GJV84_RS22110 4616945..4617256 + 312 WP_009652410.1 hypothetical protein -
GJV84_RS22115 4617303..4617773 + 471 WP_009652431.1 hypothetical protein -
GJV84_RS22120 4617822..4618334 + 513 WP_009652395.1 DUF4234 domain-containing protein -
GJV84_RS22125 4618417..4618638 + 222 WP_009652443.1 hypothetical protein -
GJV84_RS22130 4618694..4618927 + 234 WP_009652474.1 DUF905 domain-containing protein -
GJV84_RS22135 4619031..4619849 + 819 WP_009652424.1 DUF945 domain-containing protein -
GJV84_RS22140 4619849..4620103 + 255 WP_009652519.1 hypothetical protein -
GJV84_RS22145 4620120..4620593 + 474 WP_080336072.1 antirestriction protein -
GJV84_RS22150 4620604..4620822 + 219 WP_009652503.1 hypothetical protein -
GJV84_RS22155 4620834..4621316 + 483 WP_016154455.1 DNA repair protein RadC -
GJV84_RS22160 4621330..4621551 + 222 WP_009652392.1 DUF987 domain-containing protein -
GJV84_RS22165 4621608..4621934 + 327 WP_162838704.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GJV84_RS22170 4621984..4622361 + 378 WP_016154457.1 TA system toxin CbtA family protein Toxin
GJV84_RS22175 4622358..4622849 + 492 WP_009652438.1 hypothetical protein -
GJV84_RS22180 4622879..4623082 + 204 WP_009652505.1 DUF957 domain-containing protein -
GJV84_RS22185 4623163..4624008 + 846 Protein_4346 DUF4942 domain-containing protein -
GJV84_RS22190 4624020..4624301 + 282 WP_071687634.1 hypothetical protein -
GJV84_RS22195 4624688..4626349 + 1662 WP_071685461.1 fatty acid--CoA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(4-103)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14168.19 Da        Isoelectric Point: 9.5224

>T145937 WP_016154457.1 NZ_CP047307:4621984-4622361 [Citrobacter freundii]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Download         Length: 378 bp

>T145937 NZ_CP062711:3114579-3114686 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12079.85 Da        Isoelectric Point: 6.6283

>AT145937 WP_162838704.1 NZ_CP047307:4621608-4621934 [Citrobacter freundii]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Download         Length: 327 bp

>AT145937 NZ_CP062711:c3114531-3114472 [Escherichia coli O157:H7]
GTCTGGTTTCAAGATTAGCCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R8WN26

Antitoxin

Source ID Structure

References