Detailed information of TA system TA145935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4383994..4385544
• Replicon: chromosome
• Accession: NZ_CP047307
• Organism: Citrobacter freundii strain L75

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GJV84_RS20955
• Protein ID: WP_032724016.1
• Coordinates: 4383994..4385289 (-)
• Length: 432 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5P2M8A1
• Locus tag: GJV84_RS20960
• Protein ID: WP_032724128.1
• Coordinates: 4385293..4385544 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJV84_RS20925	4379016..4379921	+	906	WP_032724022.1	DUF1738 domain-containing protein	-
GJV84_RS20930	4379986..4380357	+	372	WP_071684603.1	hypothetical protein	-
GJV84_RS20935	4380531..4382120	+	1590	WP_071684602.1	TraI domain-containing protein	-
GJV84_RS25775	4382144..4382821	-	678	WP_071684601.1	HNH endonuclease	-
GJV84_RS20945	4382875..4383528	-	654	WP_071684600.1	HNH endonuclease	-
GJV84_RS20950	4383640..4383906	-	267	Protein_4102	hypothetical protein	-
GJV84_RS20955	4383994..4385289	-	1296	WP_032724016.1	type II toxin-antitoxin system HipA family toxin	Toxin
GJV84_RS20960	4385293..4385544	-	252	WP_032724128.1	helix-turn-helix domain-containing protein	Antitoxin
GJV84_RS20965	4386185..4387528	+	1344	WP_032724015.1	DUF4433 domain-containing protein	-
GJV84_RS20970	4388221..4389336	+	1116	WP_032724014.1	macro domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 432 a.a.
• Molecular weight: 49236.59 Da
• Isoelectric Point: 6.8305

>T145935 WP_032724016.1 NZ_CP047307:c4385289-4383994 [Citrobacter freundii]
VERLTVALNGIIVGTLEKTAGGEMSFYYQRDWLERAGSRAISLSMPLREERYRGEIVYNFFDNLLPDSEQIRSRIQARFH
APTKQPFDLLARIGMDCVGAIQLWPYETDIPSIQRVDAEPLTENEIEHLLQGYKDAPLGMQAEVDDFRISLAGAQEKTAL
LWWNNRWCRPRGSTPTSHIFKLPIGYIANNGIDLRESVENEWLCSKIAAEFGFPVAETRVAYFGQQKILIVERFDRRWAK
DGQWLMRLPTEDFCQALGVAPALKYESDGGPGIARSMDLLLGSQQPLEDRILFFRSQIFFWMLAAIDGHAKNFSVFLEPN
SAYRMAPLYDVMSAYPLMHPKGIPPRKAKMAMALSGRNRHFHWSTIMPRHFLSTAREVSFPVLQVEQLLAEMKQRTESVI
SKVETMLPAGFPEHISEAIFEGLRHQSARIP

Nucleotide

• Length: 1296 bp

>T145935 NZ_CP062711:c2856679-2856503 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9506.81 Da
• Isoelectric Point: 9.9484

>AT145935 WP_032724128.1 NZ_CP047307:c4385544-4385293 [Citrobacter freundii]
MNVTSPKMLAQAVRDARKQRKLTQREAAESMSMKQSTVSEFENHPEGTRLDTLFKLLAALDLELQVVSRKGKNKTSEGWD
QEW

Nucleotide

• Length: 252 bp

>AT145935 NZ_CP062711:c2856457-2856041 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5P2M8A1