Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3922775..3923454 Replicon chromosome
Accession NZ_CP047307
Organism Citrobacter freundii strain L75

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag GJV84_RS18795 Protein ID WP_003031349.1
Coordinates 3923113..3923454 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag GJV84_RS18790 Protein ID WP_003031347.1
Coordinates 3922775..3923092 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV84_RS18765 3918199..3920196 - 1998 WP_016245729.1 choline BCCT transporter BetT -
GJV84_RS18770 3920562..3921542 - 981 WP_121566969.1 IS5-like element ISKpn26 family transposase -
GJV84_RS18775 3921577..3922035 + 459 WP_159359444.1 antirestriction protein -
GJV84_RS18780 3922051..3922527 + 477 WP_003031345.1 RadC family protein -
GJV84_RS18785 3922536..3922757 + 222 WP_003031346.1 DUF987 domain-containing protein -
GJV84_RS18790 3922775..3923092 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GJV84_RS18795 3923113..3923454 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
GJV84_RS18805 3924084..3924548 - 465 WP_003843778.1 isoprenylcysteine carboxylmethyltransferase family protein -
GJV84_RS18810 3924741..3926189 - 1449 WP_003838773.1 EAL domain-containing protein -
GJV84_RS18815 3926531..3927004 - 474 WP_044702484.1 hypothetical protein -
GJV84_RS18820 3926979..3927710 - 732 WP_046669763.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3885211..3935351 50140
- flank IS/Tn - - 3920562..3921542 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T145934 WP_003031349.1 NZ_CP047307:3923113-3923454 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T145934 NZ_CP062711:2694917-2695072 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT145934 WP_003031347.1 NZ_CP047307:3922775-3923092 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT145934 NZ_CP062711:c2694905-2694847 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References