Detailed information of TA system TA145932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2426274..2426913
• Replicon: chromosome
• Accession: NZ_CP047307
• Organism: Citrobacter freundii strain L75

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: GJV84_RS11520
• Protein ID: WP_003020221.1
• Coordinates: 2426274..2426450 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GJV84_RS11525
• Protein ID: WP_123924900.1
• Coordinates: 2426497..2426913 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJV84_RS11500	2423052..2423282	-	231	WP_003020233.1	DUF2554 family protein	-
GJV84_RS11505	2423472..2423597	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
GJV84_RS11510	2423597..2424607	-	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
GJV84_RS11515	2424607..2426010	-	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
GJV84_RS11520	2426274..2426450	+	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
GJV84_RS11525	2426497..2426913	+	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GJV84_RS11530	2427006..2428415	+	1410	WP_071685591.1	PLP-dependent aminotransferase family protein	-
GJV84_RS11535	2428744..2429889	+	1146	WP_003020212.1	ABC transporter substrate-binding protein	-
GJV84_RS11540	2429906..2430922	+	1017	WP_058842662.1	ABC transporter ATP-binding protein	-
GJV84_RS11545	2430923..2431867	+	945	WP_003843727.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T145932 WP_003020221.1 NZ_CP047307:2426274-2426450 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T145932 NZ_CP062711:1094578-1094913 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT145932 WP_123924900.1 NZ_CP047307:2426497-2426913 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT145932 NZ_CP062711:1094330-1094578 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36