Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 944929..945583 Replicon chromosome
Accession NZ_CP047307
Organism Citrobacter freundii strain L75

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag GJV84_RS04615 Protein ID WP_003026936.1
Coordinates 945176..945583 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag GJV84_RS04610 Protein ID WP_003026938.1
Coordinates 944929..945195 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV84_RS04585 940129..941562 - 1434 WP_071685098.1 6-phospho-beta-glucosidase BglA -
GJV84_RS04590 941684..942412 - 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
GJV84_RS04595 942465..942776 + 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
GJV84_RS04600 942939..943598 + 660 WP_003026947.1 hemolysin III family protein -
GJV84_RS04605 943692..944672 - 981 WP_071685097.1 tRNA-modifying protein YgfZ -
GJV84_RS04610 944929..945195 + 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
GJV84_RS04615 945176..945583 + 408 WP_003026936.1 protein YgfX Toxin
GJV84_RS04620 945628..946149 - 522 WP_159359334.1 flavodoxin FldB -
GJV84_RS04625 946263..947159 + 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
GJV84_RS04630 947183..947896 + 714 WP_003825520.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GJV84_RS04635 947902..949635 + 1734 WP_054528765.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T145923 WP_003026936.1 NZ_CP047307:945176-945583 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T145923 NZ_CP062710:c26341-26081 [Escherichia coli O157:H7]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTATTTTTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT145923 WP_003026938.1 NZ_CP047307:944929-945195 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT145923 NZ_CP062710:c26091-25849 [Escherichia coli O157:H7]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTCCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References