Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 851878..852431 Replicon chromosome
Accession NZ_CP047307
Organism Citrobacter freundii strain L75

Toxin (Protein)


Gene name ccdB Uniprot ID A0A443VE20
Locus tag GJV84_RS04125 Protein ID WP_057108625.1
Coordinates 852117..852431 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A443VE25
Locus tag GJV84_RS04120 Protein ID WP_057108464.1
Coordinates 851878..852114 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV84_RS04090 847905..848255 - 351 WP_009309933.1 IS66 family insertion sequence element accessory protein TnpB -
GJV84_RS04095 848252..848656 - 405 WP_009309932.1 IS66 family insertion sequence hypothetical protein -
GJV84_RS04100 849289..849507 - 219 Protein_806 transposase -
GJV84_RS04105 849440..849736 - 297 WP_057108462.1 hypothetical protein -
GJV84_RS04110 850371..850628 - 258 WP_140415848.1 hypothetical protein -
GJV84_RS04115 850611..851555 + 945 WP_057108463.1 DUF1738 domain-containing protein -
GJV84_RS04120 851878..852114 + 237 WP_057108464.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
GJV84_RS04125 852117..852431 + 315 WP_057108625.1 CcdB family protein Toxin
GJV84_RS04130 852428..853180 - 753 WP_064388594.1 hypothetical protein -
GJV84_RS04135 853189..854925 - 1737 WP_064388593.1 type IV secretory system conjugative DNA transfer family protein -
GJV84_RS25700 855276..855635 - 360 WP_072146388.1 hypothetical protein -
GJV84_RS04145 855701..856006 - 306 WP_072146387.1 dpoa decarboxylase -
GJV84_RS04150 856055..856357 - 303 WP_057108600.1 conjugal transfer protein -
GJV84_RS04155 856406..856804 - 399 WP_057108599.1 cag pathogenicity island Cag12 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - rfaE / hcp2/tssD2 / AHA_1833 / fha / tssJ / tssL / clpV/tssH / tssA / tssA 691445..863473 172028


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(10-78)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11487.35 Da        Isoelectric Point: 7.9888

>T145922 WP_057108625.1 NZ_CP047307:852117-852431 [Citrobacter freundii]
MQFTVYGNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPVDRYPAGRRPDRLVPVVRLTDGQEYAVMTHELASVPVRALG
AVFCDASQYRTQVKAAIDFLIDGI

Download         Length: 315 bp

>T145922 NZ_CP062710:c24311-24129 [Escherichia coli O157:H7]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9012.08 Da        Isoelectric Point: 6.2206

>AT145922 WP_057108464.1 NZ_CP047307:851878-852114 [Citrobacter freundii]
MTAKQRNTLSVTMTVERALLVRAREAGINLSATLTAALDAELRHHEAKKWQEENREAIDALNRFHDEYGCFSDEYRTF

Download         Length: 237 bp

>AT145922 NZ_CP062710:c24104-23667 [Escherichia coli O157:H7]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATTGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAGCTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A443VE20

Antitoxin

Source ID Structure
AlphaFold DB A0A443VE25

References