Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 811412..812190 Replicon chromosome
Accession NZ_CP047307
Organism Citrobacter freundii strain L75

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A443VCT1
Locus tag GJV84_RS03920 Protein ID WP_057108449.1
Coordinates 811702..812190 (+) Length 163 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A1Y0L5F0
Locus tag GJV84_RS03915 Protein ID WP_038917748.1
Coordinates 811412..811705 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV84_RS03895 806915..808282 + 1368 WP_044713776.1 type VI secretion system ImpA and VasL domain-containing protein -
GJV84_RS03900 808285..808764 + 480 WP_044713777.1 Hcp family type VI secretion system effector -
GJV84_RS03905 808965..809822 - 858 WP_159359332.1 hypothetical protein -
GJV84_RS03910 810236..811014 + 779 Protein_768 IS5 family transposase -
GJV84_RS03915 811412..811705 + 294 WP_038917748.1 DUF1778 domain-containing protein Antitoxin
GJV84_RS03920 811702..812190 + 489 WP_057108449.1 GNAT family N-acetyltransferase Toxin
GJV84_RS03925 812283..813386 + 1104 WP_064388374.1 hypothetical protein -
GJV84_RS03930 813662..814151 - 490 Protein_772 molybdopterin-guanine dinucleotide biosynthesis protein MobC -
GJV84_RS03935 814722..816131 + 1410 WP_057108451.1 N-6 DNA methylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - rfaE / hcp2/tssD2 / AHA_1833 / fha / tssJ / tssL / clpV/tssH / tssA / tssA 691445..863473 172028


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(71-143)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 163 a.a.        Molecular weight: 17572.45 Da        Isoelectric Point: 7.2150

>T145921 WP_057108449.1 NZ_CP047307:811702-812190 [Citrobacter freundii]
MISAPEPLRAEHVLSSFCCGVESMDNWLKLRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVAMNSAPGRFRRNMPDPI
PVVMLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SF

Download         Length: 489 bp

>T145921 NZ_CP062709:71588-71746 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10872.44 Da        Isoelectric Point: 10.1968

>AT145921 WP_038917748.1 NZ_CP047307:811412-811705 [Citrobacter freundii]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPSPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT145921 NZ_CP062709:71325-71523 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A443VCT1

Antitoxin

Source ID Structure
AlphaFold DB A0A1Y0L5F0

References