Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 260797..261428 Replicon chromosome
Accession NZ_CP047307
Organism Citrobacter freundii strain L75

Toxin (Protein)


Gene name hicA Uniprot ID A0A0J1MPQ6
Locus tag GJV84_RS01215 Protein ID WP_003837806.1
Coordinates 260797..261072 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0J1MMS0
Locus tag GJV84_RS01220 Protein ID WP_003837808.1
Coordinates 261069..261428 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV84_RS01205 256848..259595 + 2748 WP_049025788.1 ribosome-associated ATPase/putative transporter RbbA -
GJV84_RS01210 259595..260719 + 1125 WP_003837801.1 ABC transporter permease -
GJV84_RS01215 260797..261072 + 276 WP_003837806.1 type II toxin-antitoxin system HicA family toxin Toxin
GJV84_RS01220 261069..261428 + 360 WP_003837808.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GJV84_RS01225 261541..261942 - 402 WP_003023387.1 nickel-responsive transcriptional regulator NikR -
GJV84_RS01230 261992..262570 - 579 WP_003837810.1 4'-phosphopantetheinyl transferase AcpT -
GJV84_RS01235 262633..263682 - 1050 WP_003023393.1 AI-2E family transporter -
GJV84_RS01240 263816..265033 + 1218 WP_071684567.1 MFS transporter -
GJV84_RS01245 265037..265594 - 558 WP_003837816.1 DcrB family lipoprotein -
GJV84_RS01250 265667..266332 - 666 WP_003837818.1 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10210.88 Da        Isoelectric Point: 10.7228

>T145919 WP_003837806.1 NZ_CP047307:260797-261072 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Download         Length: 276 bp

>T145919 NZ_CP062709:2065-2340 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13198.98 Da        Isoelectric Point: 4.5462

>AT145919 WP_003837808.1 NZ_CP047307:261069-261428 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Download         Length: 360 bp

>AT145919 NZ_CP062709:1781-2065 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MPQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MMS0

References