Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 317109..317875 Replicon chromosome
Accession NZ_CP047306
Organism Vibrio cholerae O37 strain ATCC 25872

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag GTH07_RS14710 Protein ID WP_000982260.1
Coordinates 317109..317606 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag GTH07_RS14715 Protein ID WP_000246253.1
Coordinates 317603..317875 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH07_RS14670 (GTH07_14680) 312265..312618 + 354 WP_001138366.1 50S ribosomal protein L20 -
GTH07_RS14675 (GTH07_14685) 312700..313662 - 963 WP_000841995.1 integron integrase IntIA -
GTH07_RS18540 314073..314156 + 84 WP_228840824.1 DUF645 family protein -
GTH07_RS14685 (GTH07_14695) 314475..314978 + 504 WP_001158944.1 DinB family protein -
GTH07_RS18550 (GTH07_14700) 315035..315097 + 63 Protein_263 acetyltransferase -
GTH07_RS14690 (GTH07_14705) 315160..315465 - 306 WP_000140489.1 hypothetical protein -
GTH07_RS14695 (GTH07_14710) 315493..315915 - 423 WP_000613531.1 winged helix-turn-helix domain-containing protein -
GTH07_RS18555 316050..316206 + 157 Protein_266 acetyltransferase -
GTH07_RS18560 316338..316400 + 63 Protein_267 DUF645 family protein -
GTH07_RS18565 316387..316488 + 102 WP_227746414.1 Tfp pilus assembly protein -
GTH07_RS14705 (GTH07_14720) 316668..316961 + 294 WP_125460920.1 hypothetical protein -
GTH07_RS14710 (GTH07_14725) 317109..317606 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
GTH07_RS14715 (GTH07_14730) 317603..317875 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
GTH07_RS14720 (GTH07_14735) 318267..318446 + 180 WP_001883058.1 DUF645 family protein -
GTH07_RS14725 (GTH07_14740) 318671..319783 + 1113 WP_212563483.1 AAA family ATPase -
GTH07_RS14730 (GTH07_14745) 319788..320450 + 663 WP_001064760.1 RelA/SpoT domain-containing protein -
GTH07_RS14735 (GTH07_14750) 320620..320973 + 354 WP_000384364.1 DUF6404 family protein -
GTH07_RS14740 (GTH07_14755) 321125..321511 + 387 WP_001015080.1 MmcQ/YjbR family DNA-binding protein -
GTH07_RS14745 (GTH07_14760) 321711..321992 + 282 WP_000086651.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GTH07_RS14750 (GTH07_14765) 321989..322276 + 288 WP_000869993.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 307620..433724 126104
- inside Integron - - 312700..433348 120648


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T145903 WP_000982260.1 NZ_CP047306:c317606-317109 [Vibrio cholerae O37]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T145903 NZ_CP062708:2694906-2695061 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT145903 WP_000246253.1 NZ_CP047306:c317875-317603 [Vibrio cholerae O37]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT145903 NZ_CP062708:c2694894-2694836 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
PDB 1Y9B
AlphaFold DB A0A0F2IC79

References