Detailed information of TA system TA145902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2219593..2221418
• Replicon: chromosome
• Accession: NZ_CP047305
• Organism: Vibrio cholerae O37 strain ATCC 25872

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GTH07_RS10005
• Protein ID: WP_000391994.1
• Coordinates: 2219593..2220954 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GTH07_RS10010
• Protein ID: WP_000804849.1
• Coordinates: 2220951..2221418 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH07_RS09990 (GTH07_09985)	2215446..2216966	+	1521	WP_000640055.1	aldehyde dehydrogenase family protein	-
GTH07_RS09995 (GTH07_09990)	2217308..2217988	+	681	Protein_1940	IS5 family transposase	-
GTH07_RS10000 (GTH07_09995)	2218014..2218991	-	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
GTH07_RS10005 (GTH07_10000)	2219593..2220954	+	1362	WP_000391994.1	type II toxin-antitoxin system HipA family toxin	Toxin
GTH07_RS10010 (GTH07_10005)	2220951..2221418	+	468	WP_000804849.1	helix-turn-helix transcriptional regulator	Antitoxin
GTH07_RS10015 (GTH07_10010)	2221846..2222424	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
GTH07_RS10020 (GTH07_10015)	2222428..2225364	+	2937	WP_001283870.1	DEAD/DEAH box helicase family protein	-
GTH07_RS10025 (GTH07_10020)	2225434..2226137	+	704	Protein_1946	nuclease-related domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51319.16 Da
• Isoelectric Point: 4.8586

>T145902 WP_000391994.1 NZ_CP047305:2219593-2220954 [Vibrio cholerae O37]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRETELNYLSDYALEHYDKDDFHAVSLNHPVSFFFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTISPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSNHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T145902 NZ_CP062708:2364669-2364772 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT145902 WP_000804849.1 NZ_CP047305:2220951-2221418 [Vibrio cholerae O37]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT145902 NZ_CP062708:c2364810-2364539 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT