Detailed information of TA system TA145862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2207153..2208978
• Replicon: chromosome
• Accession: NZ_CP047301
• Organism: Vibrio cholerae O1 biovar El Tor strain DRC-193A

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: GTF75_RS09940
• Protein ID: WP_000392003.1
• Coordinates: 2207153..2208514 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GTF75_RS09945
• Protein ID: WP_000804849.1
• Coordinates: 2208511..2208978 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF75_RS09925	2203004..2204524	+	1521	WP_000640055.1	aldehyde dehydrogenase	-
GTF75_RS09930	2204866..2205546	+	681	Protein_1927	IS5 family transposase	-
GTF75_RS09935	2205572..2206549	-	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
GTF75_RS09940	2207153..2208514	+	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
GTF75_RS09945	2208511..2208978	+	468	WP_000804849.1	helix-turn-helix domain-containing protein	Antitoxin
GTF75_RS09950	2209406..2209984	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
GTF75_RS09955	2209988..2212924	+	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
GTF75_RS09960	2212994..2213698	+	705	WP_001880605.1	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	acfA / acfC / acfB / tcpJ / tcpN/toxT / tcpF / tcpE / tcpT / tcpS / tcpD / tcpR / tcpC / tcpQ / tcpB / tcpA / tcpH / tcpP / tcpI	2173590..2214541	40951
	-	flank	IS/Tn	-	-	2205572..2206324	752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T145862 WP_000392003.1 NZ_CP047301:2207153-2208514 [Vibrio cholerae O1 biovar El Tor]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T145862 NZ_CP062705:284691-284798 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT145862 WP_000804849.1 NZ_CP047301:2208511-2208978 [Vibrio cholerae O1 biovar El Tor]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT145862 NZ_CP062705:c284643-284577 [Escherichia coli O157:H7]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8