Detailed information of TA system TA145842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2207093..2208918
• Replicon: chromosome
• Accession: NZ_CP047299
• Organism: Vibrio cholerae O1 biovar El Tor strain P27459

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: GTF71_RS09925
• Protein ID: WP_000392003.1
• Coordinates: 2207093..2208454 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GTF71_RS09930
• Protein ID: WP_000804849.1
• Coordinates: 2208451..2208918 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF71_RS09910	2202944..2204464	+	1521	WP_000640055.1	aldehyde dehydrogenase	-
GTF71_RS09915	2204806..2205486	+	681	Protein_1927	IS5 family transposase	-
GTF71_RS09920	2205512..2206489	-	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
GTF71_RS09925	2207093..2208454	+	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
GTF71_RS09930	2208451..2208918	+	468	WP_000804849.1	helix-turn-helix domain-containing protein	Antitoxin
GTF71_RS09935	2209346..2209924	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
GTF71_RS09940	2209928..2212864	+	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
GTF71_RS09945	2212934..2213638	+	705	WP_001880605.1	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T145842 WP_000392003.1 NZ_CP047299:2207093-2208454 [Vibrio cholerae O1 biovar El Tor]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T145842 NZ_CP062702:2622444-2622599 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT145842 WP_000804849.1 NZ_CP047299:2208451-2208918 [Vibrio cholerae O1 biovar El Tor]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT145842 NZ_CP062702:c2622432-2622374 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8