Detailed information of TA system TA145822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2263151..2264976
• Replicon: chromosome
• Accession: NZ_CP047297
• Organism: Vibrio cholerae O1 biovar El Tor strain C6709

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: GTF73_RS10240
• Protein ID: WP_000392003.1
• Coordinates: 2263151..2264512 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GTF73_RS10245
• Protein ID: WP_000804849.1
• Coordinates: 2264509..2264976 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF73_RS10225	2259002..2260522	+	1521	WP_000640055.1	aldehyde dehydrogenase	-
GTF73_RS10230	2260864..2261544	+	681	Protein_1983	IS5 family transposase	-
GTF73_RS10235	2261570..2262547	-	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
GTF73_RS10240	2263151..2264512	+	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
GTF73_RS10245	2264509..2264976	+	468	WP_000804849.1	helix-turn-helix domain-containing protein	Antitoxin
GTF73_RS10250	2265404..2265982	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
GTF73_RS10255	2265986..2268922	+	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
GTF73_RS10260	2268992..2269696	+	705	WP_001880605.1	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T145822 WP_000392003.1 NZ_CP047297:2263151-2264512 [Vibrio cholerae O1 biovar El Tor]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T145822 NZ_CP062700:c4448401-4448003 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT145822 WP_000804849.1 NZ_CP047297:2264509-2264976 [Vibrio cholerae O1 biovar El Tor]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT145822 NZ_CP062700:c4448697-4448404 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8