Detailed information of TA system TA145802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2257965..2259790
• Replicon: chromosome
• Accession: NZ_CP047295
• Organism: Vibrio cholerae C6706

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: GTF72_RS10215
• Protein ID: WP_000392003.1
• Coordinates: 2257965..2259326 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GTF72_RS10220
• Protein ID: WP_000804849.1
• Coordinates: 2259323..2259790 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF72_RS10200	2253816..2255336	+	1521	WP_000640055.1	aldehyde dehydrogenase	-
GTF72_RS10205	2255678..2256358	+	681	Protein_1978	IS5 family transposase	-
GTF72_RS10210	2256384..2257361	-	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
GTF72_RS10215	2257965..2259326	+	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
GTF72_RS10220	2259323..2259790	+	468	WP_000804849.1	helix-turn-helix domain-containing protein	Antitoxin
GTF72_RS10225	2260218..2260796	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
GTF72_RS10230	2260800..2263736	+	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
GTF72_RS10235	2263806..2264510	+	705	WP_001880605.1	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T145802 WP_000392003.1 NZ_CP047295:2257965-2259326 [Vibrio cholerae C6706]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T145802 NZ_CP062700:277238-277345 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT145802 WP_000804849.1 NZ_CP047295:2259323-2259790 [Vibrio cholerae C6706]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT145802 NZ_CP062700:c277190-277124 [Escherichia coli O157:H7]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8