Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 52213..52856 Replicon plasmid pKp4101
Accession NZ_CP047282
Organism Klebsiella quasipneumoniae strain N18-04101

Toxin (Protein)


Gene name vagD Uniprot ID A0A6C0NE46
Locus tag F3109_RS26115 Protein ID WP_008322233.1
Coordinates 52213..52629 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag F3109_RS26120 Protein ID WP_001261276.1
Coordinates 52626..52856 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3109_RS26095 48283..49302 + 1020 WP_004113181.1 ribose ABC transporter permease -
F3109_RS26100 49380..50750 + 1371 WP_008322237.1 hypothetical protein -
F3109_RS26105 50747..51667 + 921 WP_008322235.1 carbohydrate kinase -
F3109_RS26110 51850..52019 - 170 Protein_69 DUF596 domain-containing protein -
F3109_RS26115 52213..52629 - 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin Toxin
F3109_RS26120 52626..52856 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
F3109_RS26125 53109..54131 - 1023 WP_008322227.1 helicase UvrD -
F3109_RS26130 54217..54921 + 705 WP_001067848.1 IS6-like element IS26 family transposase -
F3109_RS26135 55324..55680 + 357 WP_000215515.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-204 / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / catA2 / blaTEM-1B / aac(3)-IId / blaCMY-4 - 1..187068 187068


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15054.58 Da        Isoelectric Point: 9.2957

>T145785 WP_008322233.1 NZ_CP047282:c52629-52213 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAASAVLVTNNVREFARVPGLMLEDWVK

Download         Length: 417 bp

>T145785 NZ_CP062473:c1504843-1504661 [Lentilactobacillus parabuchneri]
ATGGACTCCAGAACCATCATCCAGATGTTAAAACATGATGGGTGGTACAAAGTTAGAATCCATGGGGACCATTGGCATTT
TAAACACCCTCAAAAGCTGGGGATTGTAACTGTGCCACATCCTCGTAAAGATATTCCAAAAGGAACGTACAATAGCATTC
TAAAACAAGCGGGGCTCAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT145785 WP_001261276.1 NZ_CP047282:c52856-52626 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT145785 NZ_CP062473:c1504625-1504227 [Lentilactobacillus parabuchneri]
ATGAAATATTTGTATTATGCTTTATTCAAAAAGCAATCAGATGACAAAATTGAAGTTACATTCCCTGACTTAGCCCCCCA
CGCAGCAACATATGGAACAACGATGTCAGATGCCCTTCATATGGCTCATGATGCCTTGGAAGGCTACTTATTAGTTGTTG
AGGATTTACACGAGAAGCTGCCTAAGGCTTCAAGTCCAGAATCATTAACACATGATACAAGCCAGCTGATCATTCCTATC
GAGGTCGACACAACGTTGGCACGGGAACGTGAAGACAATGTAAGTATCAAAAAGACGTTAACAATTCCAAAATATTTAAA
TGATTTAGGCAAGCAAAAAAGCATCAATTTCTCTGCCACACTCACGGATGCCTTAAAGCACAAGCTCAATATTTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE46

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References