Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | kacAT/ElaA-DUF1778 |
Location | 192525..193328 | Replicon | plasmid pCf4128 |
Accession | NZ_CP047280 | ||
Organism | Citrobacter freundii strain N18-04128 |
Toxin (Protein)
Gene name | KacT | Uniprot ID | A0A7W3E3C9 |
Locus tag | F3113_RS26365 | Protein ID | WP_022652313.1 |
Coordinates | 192798..193328 (+) | Length | 177 a.a. |
Antitoxin (Protein)
Gene name | KacA | Uniprot ID | W8E6Q5 |
Locus tag | F3113_RS26360 | Protein ID | WP_022652312.1 |
Coordinates | 192525..192794 (+) | Length | 90 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
F3113_RS26345 | 188830..189137 | - | 308 | Protein_218 | IS1 family transposase | - |
F3113_RS26350 | 189614..190354 | + | 741 | WP_022652310.1 | site-specific integrase | - |
F3113_RS26355 | 190680..191669 | - | 990 | WP_022652311.1 | RepB family plasmid replication initiator protein | - |
F3113_RS26360 | 192525..192794 | + | 270 | WP_022652312.1 | DUF1778 domain-containing protein | Antitoxin |
F3113_RS26365 | 192798..193328 | + | 531 | WP_022652313.1 | GNAT family N-acetyltransferase | Toxin |
F3113_RS26370 | 193460..194353 | + | 894 | WP_077873527.1 | IS5 family transposase | - |
F3113_RS26375 | 194494..195390 | - | 897 | Protein_224 | IS3 family transposase | - |
F3113_RS26380 | 195455..196606 | + | 1152 | WP_022652315.1 | IS30-like element IS30 family transposase | - |
F3113_RS26385 | 196715..197098 | + | 384 | WP_001201739.1 | IS66 family insertion sequence hypothetical protein | - |
F3113_RS26390 | 197095..197442 | + | 348 | WP_000609174.1 | IS66 family insertion sequence element accessory protein TnpB | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Conjugative plasmid | sul2 / blaCMY-4 / blaOXA-204 / aph(3'')-Ib / aph(6)-Id / tet(A) / catA2 / blaTEM-1B / aac(3)-IId / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr | - | 1..262422 | 262422 | |
- | inside | IScluster/Tn | - | - | 187516..200897 | 13381 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 177 a.a. Molecular weight: 20349.26 Da Isoelectric Point: 6.7496
>T145770 WP_022652313.1 NZ_CP047280:192798-193328 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE
Download Length: 531 bp
>T145770 NZ_CP062469:989476-989727 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCCTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGGTTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG
ATGAATAACCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCCTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGGTTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG
Antitoxin
Download Length: 90 a.a. Molecular weight: 10269.70 Da Isoelectric Point: 5.7252
>AT145770 WP_022652312.1 NZ_CP047280:192525-192794 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE
Download Length: 270 bp
>AT145770 NZ_CP062469:989717-989890 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7W3E3C9 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A2Z3XDS5 |