145765

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4116814..4117493 Replicon chromosome
Accession NZ_CP047279
Organism Citrobacter freundii strain N18-04128

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag F3113_RS20020 Protein ID WP_003031349.1
Coordinates 4117152..4117493 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag F3113_RS20015 Protein ID WP_003031347.1
Coordinates 4116814..4117131 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3113_RS19990 4112907..4114904 - 1998 WP_016245729.1 choline BCCT transporter BetT -
F3113_RS19995 4115408..4115599 + 192 Protein_3938 DUF905 family protein -
F3113_RS20000 4115613..4116074 + 462 WP_003031344.1 antirestriction protein -
F3113_RS20005 4116090..4116566 + 477 WP_003031345.1 RadC family protein -
F3113_RS20010 4116575..4116796 + 222 WP_003031346.1 DUF987 domain-containing protein -
F3113_RS20015 4116814..4117131 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
F3113_RS20020 4117152..4117493 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
F3113_RS20025 4118083..4118574 - 492 WP_121927018.1 tail fiber assembly protein -
F3113_RS20030 4119695..4120138 - 444 Protein_3945 phage tail protein -
F3113_RS20035 4120138..4120818 - 681 WP_121927014.1 DUF2612 domain-containing protein -
F3113_RS20040 4120815..4122014 - 1200 WP_121927013.1 baseplate J/gp47 family protein -
F3113_RS20045 4122015..4122368 - 354 WP_016239889.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4115613..4159949 44336


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T145765 WP_003031349.1 NZ_CP047279:4117152-4117493 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T145765 NZ_CP062469:527535-527801 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT145765 WP_003031347.1 NZ_CP047279:4116814-4117131 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT145765 NZ_CP062469:527278-527535 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References