Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 304386..305017 Replicon chromosome
Accession NZ_CP047279
Organism Citrobacter freundii strain N18-04128

Toxin (Protein)


Gene name hicA Uniprot ID A0A0J1MPQ6
Locus tag F3113_RS01425 Protein ID WP_003837806.1
Coordinates 304386..304661 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0J1MMS0
Locus tag F3113_RS01430 Protein ID WP_003837808.1
Coordinates 304658..305017 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3113_RS01415 300437..303184 + 2748 WP_044701369.1 ribosome-associated ATPase/putative transporter RbbA -
F3113_RS01420 303184..304308 + 1125 WP_003837801.1 ABC transporter permease -
F3113_RS01425 304386..304661 + 276 WP_003837806.1 type II toxin-antitoxin system HicA family toxin Toxin
F3113_RS01430 304658..305017 + 360 WP_003837808.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F3113_RS01435 305128..305529 - 402 WP_003023387.1 nickel-responsive transcriptional regulator NikR -
F3113_RS01440 305579..306157 - 579 WP_003837810.1 4'-phosphopantetheinyl transferase AcpT -
F3113_RS01445 306220..307269 - 1050 WP_003023393.1 AI-2E family transporter -
F3113_RS01450 307403..308620 + 1218 WP_003846164.1 MFS transporter -
F3113_RS01455 308624..309181 - 558 WP_003837816.1 DcrB family lipoprotein -
F3113_RS01460 309254..309919 - 666 WP_044701371.1 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10210.88 Da        Isoelectric Point: 10.7228

>T145753 WP_003837806.1 NZ_CP047279:304386-304661 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Download         Length: 276 bp

>T145753 NZ_CP062467:1220324-1220590 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13198.98 Da        Isoelectric Point: 4.5462

>AT145753 WP_003837808.1 NZ_CP047279:304658-305017 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Download         Length: 360 bp

>AT145753 NZ_CP062467:1220067-1220324 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MPQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MMS0

References