Detailed information of TA system TA145752

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 63631..64352
• Replicon: chromosome
• Accession: NZ_CP047279
• Organism: Citrobacter freundii strain N18-04128

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: F3113_RS00325
• Protein ID: WP_047399350.1
• Coordinates: 63631..63972 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0A0FFR4
• Locus tag: F3113_RS00330
• Protein ID: WP_045265421.1
• Coordinates: 64008..64352 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3113_RS00290	59057..59431	-	375	WP_016151201.1	hypothetical protein	-
F3113_RS00295	59837..60013	-	177	WP_044700470.1	hypothetical protein	-
F3113_RS00300	60131..60421	-	291	WP_044700468.1	SymE family type I addiction module toxin	-
F3113_RS00305	60558..60752	+	195	WP_044700466.1	toxin-antitoxin system HicB family antitoxin	-
F3113_RS00310	60761..61327	-	567	WP_044700465.1	hypothetical protein	-
F3113_RS00315	61558..62595	-	1038	WP_045265419.1	virulence RhuM family protein	-
F3113_RS00320	62683..63516	-	834	WP_045265420.1	DUF4942 domain-containing protein	-
F3113_RS00325	63631..63972	-	342	WP_047399350.1	TA system toxin CbtA family protein	Toxin
F3113_RS00330	64008..64352	-	345	WP_045265421.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F3113_RS00335	64376..64597	-	222	WP_045265422.1	DUF987 family protein	-
F3113_RS00340	64608..65081	-	474	WP_045265423.1	DNA repair protein RadC	-
F3113_RS00345	65152..65973	-	822	WP_183273566.1	DUF945 domain-containing protein	-
F3113_RS00350	66045..66494	-	450	WP_045265425.1	hypothetical protein	-
F3113_RS00355	66726..67529	-	804	WP_045265426.1	hypothetical protein	-
F3113_RS00360	67549..67983	-	435	WP_045265427.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (14-113)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12748.67 Da
• Isoelectric Point: 8.5196

>T145752 WP_047399350.1 NZ_CP047279:c63972-63631 [Citrobacter freundii]
MNTSPATNQRVAKPCPSPVAVWQMLLTRLLAQHYGLALSDTPFCDETVIQEHINAGVTLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQAMGLLRQGHNLSTR

Nucleotide

• Length: 342 bp

>T145752 NZ_CP062467:c85101-84784 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGTATCAGATGACGCACAAAAATTAAAAATCACAGAGTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12867.88 Da
• Isoelectric Point: 8.1459

>AT145752 WP_045265421.1 NZ_CP047279:c64352-64008 [Citrobacter freundii]
MKKDTANEVVDTLRWGLKCDITPHFGARLVQEGNRLHYLADRASIIGPFSKADLRHLNQAFPLLMKQMELMLTSGELNSR
HQHCVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 345 bp

>AT145752 NZ_CP062467:c85313-85104 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0A0FFR4