Detailed information of TA system TA145746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4028581..4029157
• Replicon: chromosome
• Accession: NZ_CP047277
• Organism: Escherichia coli strain N18-04097

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A0E2LC92
• Locus tag: F3052_RS19410
• Protein ID: WP_001299718.1
• Coordinates: 4028870..4029157 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0E2KXJ8
• Locus tag: F3052_RS19405
• Protein ID: WP_000063159.1
• Coordinates: 4028581..4028883 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3052_RS19390	4025170..4027320	+	2151	WP_001299714.1	pyruvate/proton symporter BtsT	-
F3052_RS19395	4027370..4027573	+	204	WP_000467859.1	YbdD/YjiX family protein	-
F3052_RS19400	4027584..4028540	+	957	WP_001299717.1	GTPase	-
F3052_RS19405	4028581..4028883	-	303	WP_000063159.1	BrnA antitoxin family protein	Antitoxin
F3052_RS19410	4028870..4029157	-	288	WP_001299718.1	BrnT family toxin	Toxin
F3052_RS19415	4029354..4030520	+	1167	Protein_3806	restriction endonuclease	-
F3052_RS19420	4030584..4032203	+	1620	WP_097725042.1	type I restriction-modification system subunit M	-
F3052_RS19425	4032193..4033584	+	1392	WP_000535334.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11142.60 Da
• Isoelectric Point: 7.4697

>T145746 WP_001299718.1 NZ_CP047277:c4029157-4028870 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T145746 NZ_CP062465:942958-943209 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCCTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGGTTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11536.01 Da
• Isoelectric Point: 9.4646

>AT145746 WP_000063159.1 NZ_CP047277:c4028883-4028581 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT145746 NZ_CP062465:943199-943372 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E2LC92

Antitoxin

• AlphaFold DB: A0A0E2KXJ8