Detailed information of TA system TA145740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2242225..2242751
• Replicon: chromosome
• Accession: NZ_CP047277
• Organism: Escherichia coli strain N18-04097

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: F3052_RS10770
• Protein ID: WP_000323025.1
• Coordinates: 2242464..2242751 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: F3052_RS10765
• Protein ID: WP_000534858.1
• Coordinates: 2242225..2242464 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3052_RS10745	2237587..2237943	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
F3052_RS24360	2238554..2238841	+	288	Protein_2101	hypothetical protein	-
F3052_RS10750	2239610..2240923	+	1314	WP_000589005.1	ISNCY family transposase	-
F3052_RS10755	2241360..2241692	-	333	WP_001301033.1	protein FlxA	-
F3052_RS10760	2241895..2242200	-	306	WP_001326990.1	hypothetical protein	-
F3052_RS10765	2242225..2242464	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
F3052_RS10770	2242464..2242751	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
F3052_RS10775	2242823..2242978	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
F3052_RS10780	2243195..2243446	+	252	WP_000980994.1	hypothetical protein	-
F3052_RS10785	2243513..2243791	+	279	WP_012304870.1	hypothetical protein	-
F3052_RS10790	2243793..2244842	+	1050	WP_001424248.1	DUF968 domain-containing protein	-
F3052_RS10795	2244856..2245608	+	753	WP_001047136.1	antitermination protein	-
F3052_RS10800	2245886..2245975	-	90	WP_120795389.1	hypothetical protein	-
F3052_RS10805	2246030..2246242	-	213	WP_000087756.1	cold shock-like protein CspF	-
F3052_RS10810	2246543..2246758	+	216	WP_000066484.1	cold shock-like protein CspB	-
F3052_RS10815	2247512..2247727	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2225073..2279021	53948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T145740 WP_000323025.1 NZ_CP047277:2242464-2242751 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T145740 NZ_CP062439:2743775-2744137 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT145740 WP_000534858.1 NZ_CP047277:2242225-2242464 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT145740 NZ_CP062439:2743608-2743778 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8