Detailed information of TA system TA145731

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1794566..1795398
• Replicon: chromosome
• Accession: NZ_CP047277
• Organism: Escherichia coli strain N18-04097

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: F3052_RS08450
• Protein ID: WP_000854765.1
• Coordinates: 1794566..1794940 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: F3052_RS08455
• Protein ID: WP_001295723.1
• Coordinates: 1795030..1795398 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3052_RS08415	1790025..1790354	+	330	WP_000450409.1	DUF496 family protein	-
F3052_RS08420	1790455..1790637	-	183	WP_001667686.1	ethanolamine utilization - propanediol utilization family protein	-
F3052_RS08425	1790911..1791693	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
F3052_RS08430	1791690..1792712	-	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
F3052_RS24385	1793050..1793421	-	372	WP_001445978.1	IS110 family transposase	-
F3052_RS08440	1794237..1794350	-	114	WP_001161660.1	DUF957 domain-containing protein	-
F3052_RS08445	1794363..1794569	-	207	WP_000976829.1	hypothetical protein	-
F3052_RS08450	1794566..1794940	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
F3052_RS08455	1795030..1795398	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F3052_RS08460	1795561..1795782	-	222	WP_000692345.1	DUF987 domain-containing protein	-
F3052_RS08465	1795845..1796321	-	477	WP_001186775.1	RadC family protein	-
F3052_RS08470	1796337..1796810	-	474	WP_000855064.1	antirestriction protein	-
F3052_RS08475	1797152..1797970	-	819	WP_001234729.1	DUF945 domain-containing protein	-
F3052_RS08480	1798239..1798664	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
F3052_RS08485	1798661..1799011	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T145731 WP_000854765.1 NZ_CP047277:c1794940-1794566 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T145731 NZ_CP062439:18831-19007 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT145731 WP_001295723.1 NZ_CP047277:c1795398-1795030 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT145731 NZ_CP062439:c19169-18995 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52