145725

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 212360..213163 Replicon plasmid pCf4085
Accession NZ_CP047276
Organism Citrobacter freundii strain N18-04085

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag F3084_RS26295 Protein ID WP_022652313.1
Coordinates 212633..213163 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag F3084_RS26290 Protein ID WP_022652312.1
Coordinates 212360..212629 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3084_RS26275 208665..208972 - 308 Protein_236 IS1 family transposase -
F3084_RS26280 209449..210189 + 741 WP_022652310.1 site-specific integrase -
F3084_RS26285 210515..211504 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
F3084_RS26290 212360..212629 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
F3084_RS26295 212633..213163 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
F3084_RS26300 213295..214188 + 894 WP_077873527.1 IS5 family transposase -
F3084_RS26305 214329..215225 - 897 Protein_242 IS3 family transposase -
F3084_RS26310 215290..216441 + 1152 WP_022652315.1 IS30-like element IS30 family transposase -
F3084_RS26315 216550..216933 + 384 WP_001201739.1 IS66 family insertion sequence hypothetical protein -
F3084_RS26320 216930..217277 + 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / blaCMY-4 / blaOXA-204 / aph(3'')-Ib / aph(6)-Id / tet(A) / catA2 / blaTEM-1B / aac(3)-IId / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..282258 282258
- inside IScluster/Tn - - 207351..220732 13381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T145725 WP_022652313.1 NZ_CP047276:212633-213163 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T145725 NZ_CP062421:2268404-2268670 [Staphylococcus aureus]
ATGAGCAATTATACGGTTAAGATTAAAAATTCGGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAAAAGCC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACTGTAGATGATCGAAATAAAGAAGTATTAATA
TTATCGGCGTGGTCACATTATGATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT145725 WP_022652312.1 NZ_CP047276:212360-212629 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT145725 NZ_CP062421:2268147-2268404 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGATGATTGGAAAAGTATACAAGAAACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGATAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References