145724

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 147278..147921 Replicon plasmid pCf4085
Accession NZ_CP047276
Organism Citrobacter freundii strain N18-04085

Toxin (Protein)


Gene name vagD Uniprot ID A0A6C0NE46
Locus tag F3084_RS25980 Protein ID WP_008322233.1
Coordinates 147278..147694 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag F3084_RS25985 Protein ID WP_001261276.1
Coordinates 147691..147921 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3084_RS25960 143348..144367 + 1020 WP_004113181.1 ribose ABC transporter permease -
F3084_RS25965 144445..145815 + 1371 WP_008322237.1 hypothetical protein -
F3084_RS25970 145812..146732 + 921 WP_008322235.1 carbohydrate kinase -
F3084_RS25975 146915..147084 - 170 Protein_176 DUF596 domain-containing protein -
F3084_RS25980 147278..147694 - 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin Toxin
F3084_RS25985 147691..147921 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
F3084_RS25990 148174..149196 - 1023 WP_008322227.1 helicase UvrD -
F3084_RS25995 149282..149986 + 705 WP_001067848.1 IS6-like element IS26 family transposase -
F3084_RS26000 150389..150745 + 357 WP_000215515.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / blaCMY-4 / blaOXA-204 / aph(3'')-Ib / aph(6)-Id / tet(A) / catA2 / blaTEM-1B / aac(3)-IId / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..282258 282258


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15054.58 Da        Isoelectric Point: 9.2957

>T145724 WP_008322233.1 NZ_CP047276:c147694-147278 [Citrobacter freundii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAASAVLVTNNVREFARVPGLMLEDWVK

Download         Length: 417 bp

>T145724 NZ_CP062421:1148294-1148482 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT145724 WP_001261276.1 NZ_CP047276:c147921-147691 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT145724 NZ_CP062421:1148005-1148265 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTATATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE46

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References