145721

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 4824443..4825109 Replicon chromosome
Accession NZ_CP047275
Organism Citrobacter freundii strain N18-04085

Toxin (Protein)


Gene name tad Uniprot ID A0A0D7LIM5
Locus tag F3084_RS23500 Protein ID WP_044702260.1
Coordinates 4824750..4825109 (-) Length 120 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID A0A0D7LIR0
Locus tag F3084_RS23495 Protein ID WP_003844682.1
Coordinates 4824443..4824760 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3084_RS23465 4820110..4820934 + 825 WP_003031641.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
F3084_RS23470 4821003..4822226 + 1224 WP_044702265.1 L-sorbose 1-phosphate reductase -
F3084_RS23475 4822228..4823040 + 813 WP_044702264.1 shikimate 5-dehydrogenase -
F3084_RS23480 4823092..4823448 - 357 WP_032948294.1 hypothetical protein -
F3084_RS23485 4823590..4823751 + 162 WP_003841416.1 phage protein -
F3084_RS23490 4824155..4824433 + 279 WP_044702262.1 putative addiction module antidote protein -
F3084_RS23495 4824443..4824760 - 318 WP_003844682.1 helix-turn-helix domain-containing protein Antitoxin
F3084_RS23500 4824750..4825109 - 360 WP_044702260.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F3084_RS23505 4825323..4826012 + 690 WP_003841421.1 dipeptidase PepE -
F3084_RS23510 4826078..4827709 - 1632 WP_032938230.1 Na/Pi cotransporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(14-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(12-90)


Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 13437.49 Da        Isoelectric Point: 10.2555

>T145721 WP_044702260.1 NZ_CP047275:c4825109-4824750 [Citrobacter freundii]
MTKPLYWVGQARKDLIAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNAWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Download         Length: 360 bp

>T145721 NZ_CP062421:18831-19007 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11466.35 Da        Isoelectric Point: 9.0005

>AT145721 WP_003844682.1 NZ_CP047275:c4824760-4824443 [Citrobacter freundii]
MSHDVETSSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Download         Length: 318 bp

>AT145721 NZ_CP062421:c19169-18995 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCC
GTTAAAAAGACGATGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7LIM5

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LIR0

References