Detailed information of TA system TA145720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4520985..4521501
• Replicon: chromosome
• Accession: NZ_CP047275
• Organism: Citrobacter freundii strain N18-04085

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: F3084_RS21940
• Protein ID: WP_044699148.1
• Coordinates: 4520985..4521269 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: F3084_RS21945
• Protein ID: WP_183273543.1
• Coordinates: 4521259..4521501 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3084_RS21925	4517310..4517894	+	585	WP_003839584.1	fructose PTS transporter subunit IIA	-
F3084_RS21930	4518272..4520410	+	2139	WP_003025782.1	anaerobic ribonucleoside-triphosphate reductase	-
F3084_RS21935	4520517..4520981	+	465	WP_032937736.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
F3084_RS21940	4520985..4521269	-	285	WP_044699148.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F3084_RS21945	4521259..4521501	-	243	WP_183273543.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
F3084_RS21950	4521579..4523492	-	1914	WP_003839574.1	BglG family transcription antiterminator	-
F3084_RS21955	4523514..4524254	-	741	WP_044699149.1	KDGP aldolase family protein	-
F3084_RS21960	4524251..4525369	-	1119	WP_044699152.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
F3084_RS21965	4525353..4526486	-	1134	WP_016149471.1	amidohydrolase/deacetylase family metallohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10865.69 Da
• Isoelectric Point: 10.1988

>T145720 WP_044699148.1 NZ_CP047275:c4521269-4520985 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVTVFVVAVGK
RQHSAVYLDANKRL

Nucleotide

• Length: 285 bp

>T145720 NZ_CP062420:2703281-2703580 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8947.32 Da
• Isoelectric Point: 5.0651

>AT145720 WP_183273543.1 NZ_CP047275:c4521501-4521259 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNISPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT145720 NZ_CP062420:2703069-2703278 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA