Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4087385..4088064 Replicon chromosome
Accession NZ_CP047275
Organism Citrobacter freundii strain N18-04085

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag F3084_RS19840 Protein ID WP_003031349.1
Coordinates 4087723..4088064 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag F3084_RS19835 Protein ID WP_003031347.1
Coordinates 4087385..4087702 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3084_RS19810 4083478..4085475 - 1998 WP_016245729.1 choline BCCT transporter BetT -
F3084_RS19815 4085979..4086170 + 192 Protein_3892 DUF905 family protein -
F3084_RS19820 4086184..4086645 + 462 WP_003031344.1 antirestriction protein -
F3084_RS19825 4086661..4087137 + 477 WP_003031345.1 RadC family protein -
F3084_RS19830 4087146..4087367 + 222 WP_003031346.1 DUF987 domain-containing protein -
F3084_RS19835 4087385..4087702 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
F3084_RS19840 4087723..4088064 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
F3084_RS19845 4088654..4089145 - 492 WP_121927018.1 tail fiber assembly protein -
F3084_RS19850 4090266..4090709 - 444 Protein_3899 phage tail protein -
F3084_RS19855 4090709..4091389 - 681 WP_121927014.1 DUF2612 domain-containing protein -
F3084_RS19860 4091386..4092585 - 1200 WP_121927013.1 baseplate J/gp47 family protein -
F3084_RS19865 4092586..4092939 - 354 WP_016239889.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4072428..4130520 58092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T145719 WP_003031349.1 NZ_CP047275:4087723-4088064 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T145719 NZ_CP062420:2649229-2649591 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT145719 WP_003031347.1 NZ_CP047275:4087385-4087702 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT145719 NZ_CP062420:2649062-2649232 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References