Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5025919..5026535 Replicon chromosome
Accession NZ_CP047273
Organism Citrobacter freundii strain N18-04078

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag F3085_RS24370 Protein ID WP_003028682.1
Coordinates 5025919..5026293 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag F3085_RS24375 Protein ID WP_043018956.1
Coordinates 5026293..5026535 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3085_RS24355 5023422..5024324 + 903 WP_183273563.1 formate dehydrogenase O subunit beta -
F3085_RS24360 5024321..5024956 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
F3085_RS24365 5024953..5025882 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
F3085_RS24370 5025919..5026293 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
F3085_RS24375 5026293..5026535 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
F3085_RS24380 5026741..5027649 + 909 WP_183273564.1 alpha/beta hydrolase -
F3085_RS24385 5027800..5028741 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
F3085_RS24390 5028786..5029223 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
F3085_RS24395 5029220..5030092 - 873 WP_003028669.1 virulence factor BrkB family protein -
F3085_RS24400 5030086..5030685 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T145701 WP_003028682.1 NZ_CP047273:c5026293-5025919 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T145701 NZ_CP062418:2703107-2703406 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT145701 WP_043018956.1 NZ_CP047273:c5026535-5026293 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT145701 NZ_CP062418:2702895-2703104 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References