145698

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4091328..4092007 Replicon chromosome
Accession NZ_CP047273
Organism Citrobacter freundii strain N18-04078

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag F3085_RS19845 Protein ID WP_003031349.1
Coordinates 4091666..4092007 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag F3085_RS19840 Protein ID WP_003031347.1
Coordinates 4091328..4091645 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3085_RS19815 4087421..4089418 - 1998 WP_016245729.1 choline BCCT transporter BetT -
F3085_RS19820 4089922..4090113 + 192 Protein_3898 DUF905 family protein -
F3085_RS19825 4090127..4090588 + 462 WP_003031344.1 antirestriction protein -
F3085_RS19830 4090604..4091080 + 477 WP_003031345.1 RadC family protein -
F3085_RS19835 4091089..4091310 + 222 WP_003031346.1 DUF987 domain-containing protein -
F3085_RS19840 4091328..4091645 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
F3085_RS19845 4091666..4092007 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
F3085_RS19850 4092597..4093088 - 492 WP_121927018.1 tail fiber assembly protein -
F3085_RS19855 4094209..4094652 - 444 Protein_3905 phage tail protein -
F3085_RS19860 4094652..4095332 - 681 WP_121927014.1 DUF2612 domain-containing protein -
F3085_RS19865 4095329..4096528 - 1200 WP_121927013.1 baseplate J/gp47 family protein -
F3085_RS19870 4096529..4096882 - 354 WP_016239889.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4076371..4134403 58032


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T145698 WP_003031349.1 NZ_CP047273:4091666-4092007 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T145698 NZ_CP062418:2319372-2319638 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT145698 WP_003031347.1 NZ_CP047273:4091328-4091645 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT145698 NZ_CP062418:2319121-2319372 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References