Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3855683..3856303 Replicon chromosome
Accession NZ_CP047273
Organism Citrobacter freundii strain N18-04078

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag F3085_RS18740 Protein ID WP_002892050.1
Coordinates 3856085..3856303 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag F3085_RS18735 Protein ID WP_003021733.1
Coordinates 3855683..3856057 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3085_RS18725 3850830..3852023 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
F3085_RS18730 3852046..3855195 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
F3085_RS18735 3855683..3856057 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
F3085_RS18740 3856085..3856303 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
F3085_RS18745 3856610..3857161 + 552 WP_044699372.1 maltose O-acetyltransferase -
F3085_RS18750 3857278..3857748 + 471 WP_003021724.1 YlaC family protein -
F3085_RS18755 3857827..3857967 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
F3085_RS18760 3857969..3858229 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
F3085_RS18765 3858418..3859971 + 1554 WP_044699371.1 EAL domain-containing protein -
F3085_RS18770 3860023..3860376 - 354 WP_003831033.1 DUF1428 family protein -
F3085_RS18775 3860441..3861070 - 630 WP_003835929.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T145697 WP_002892050.1 NZ_CP047273:3856085-3856303 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T145697 NZ_CP062418:2292499-2292606 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT145697 WP_003021733.1 NZ_CP047273:3855683-3856057 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT145697 NZ_CP062418:c2292683-2292623 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References