Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 207157..207960 Replicon plasmid pCf3880
Accession NZ_CP047270
Organism Citrobacter freundii strain N16-03880

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag F3114_RS26870 Protein ID WP_022652313.1
Coordinates 207430..207960 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag F3114_RS26865 Protein ID WP_022652312.1
Coordinates 207157..207426 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3114_RS26850 203462..203769 - 308 Protein_228 IS1 family transposase -
F3114_RS26855 204246..204986 + 741 WP_022652310.1 site-specific integrase -
F3114_RS26860 205312..206301 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
F3114_RS26865 207157..207426 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
F3114_RS26870 207430..207960 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
F3114_RS26875 208092..208985 + 894 WP_077873527.1 IS5 family transposase -
F3114_RS26880 209126..210022 - 897 Protein_234 IS3 family transposase -
F3114_RS26885 210087..211238 + 1152 WP_022652315.1 IS30-like element IS30 family transposase -
F3114_RS26890 211347..211730 + 384 WP_001201739.1 IS66 family insertion sequence hypothetical protein -
F3114_RS26895 211727..212074 + 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / blaCMY-4 / blaOXA-204 / catA2 / blaTEM-1B / aac(3)-IId / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..277055 277055
- inside IScluster/Tn - - 202148..215529 13381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T145661 WP_022652313.1 NZ_CP047270:207430-207960 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T145661 NZ_CP062414:2651332-2651541 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT145661 WP_022652312.1 NZ_CP047270:207157-207426 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT145661 NZ_CP062414:2651027-2651320 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References