145655

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4122652..4123331 Replicon chromosome
Accession NZ_CP047269
Organism Citrobacter freundii strain N16-03880

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag F3114_RS20100 Protein ID WP_003031349.1
Coordinates 4122990..4123331 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag F3114_RS20095 Protein ID WP_003031347.1
Coordinates 4122652..4122969 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3114_RS20070 4118745..4120742 - 1998 WP_016245729.1 choline BCCT transporter BetT -
F3114_RS20075 4121246..4121437 + 192 Protein_3938 DUF905 family protein -
F3114_RS20080 4121451..4121912 + 462 WP_003031344.1 antirestriction protein -
F3114_RS20085 4121928..4122404 + 477 WP_003031345.1 RadC family protein -
F3114_RS20090 4122413..4122634 + 222 WP_003031346.1 DUF987 domain-containing protein -
F3114_RS20095 4122652..4122969 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
F3114_RS20100 4122990..4123331 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
F3114_RS20105 4123921..4124412 - 492 WP_121927018.1 tail fiber assembly protein -
F3114_RS20110 4125533..4125976 - 444 Protein_3945 phage tail protein -
F3114_RS20115 4125976..4126656 - 681 WP_121927014.1 DUF2612 domain-containing protein -
F3114_RS20120 4126653..4127852 - 1200 WP_121927013.1 baseplate J/gp47 family protein -
F3114_RS20125 4127853..4128206 - 354 WP_016239889.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4121451..4165847 44396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T145655 WP_003031349.1 NZ_CP047269:4122990-4123331 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T145655 NZ_CP062414:c182871-182776 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT145655 WP_003031347.1 NZ_CP047269:4122652-4122969 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT145655 NZ_CP062414:182691-182748 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References