Detailed information of TA system TA145640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1726877..1727794
• Replicon: chromosome
• Accession: NZ_CP047268
• Organism: Bacillus velezensis strain DH8043

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: GKS41_RS08920
• Protein ID: WP_007407256.1
• Coordinates: 1727048..1727794 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: GKS41_RS08915
• Protein ID: WP_003154807.1
• Coordinates: 1726877..1727047 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKS41_RS08880	1722081..1723670	+	1590	WP_159354052.1	hypothetical protein	-
GKS41_RS08885	1723683..1724116	+	434	Protein_1697	hypothetical protein	-
GKS41_RS08890	1724121..1724318	+	198	WP_007610833.1	XkdX family protein	-
GKS41_RS08895	1724375..1725136	+	762	WP_159354053.1	phage portal protein	-
GKS41_RS08900	1725188..1725451	+	264	WP_003154815.1	hemolysin XhlA family protein	-
GKS41_RS08905	1725465..1725728	+	264	WP_159354054.1	phage holin	-
GKS41_RS08910	1725742..1726620	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
GKS41_RS08915	1726877..1727047	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
GKS41_RS08920	1727048..1727794	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
GKS41_RS08925	1727899..1728897	-	999	WP_003154805.1	inorganic phosphate transporter	-
GKS41_RS08930	1728910..1729527	-	618	WP_003154804.1	DUF47 domain-containing protein	-
GKS41_RS08935	1729813..1731129	-	1317	WP_007610842.1	amino acid permease	-
GKS41_RS08940	1731451..1732401	+	951	WP_159354055.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1693464..1736090	42626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T145640 WP_007407256.1 NZ_CP047268:c1727794-1727048 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T145640 NZ_CP062413:19379-19555 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGATGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT145640 WP_003154807.1 NZ_CP047268:c1727047-1726877 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT145640 NZ_CP062413:c19717-19543 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14