Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1005635..1006272 Replicon chromosome
Accession NZ_CP047268
Organism Bacillus velezensis strain DH8043

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GKS41_RS05100 Protein ID WP_003156187.1
Coordinates 1005922..1006272 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag GKS41_RS05095 Protein ID WP_003156188.1
Coordinates 1005635..1005916 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GKS41_RS05075 1002000..1002599 - 600 WP_015239195.1 rhomboid family intramembrane serine protease -
GKS41_RS05080 1002692..1003057 + 366 WP_053104569.1 holo-ACP synthase -
GKS41_RS05085 1003222..1004229 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
GKS41_RS05090 1004346..1005515 + 1170 WP_053285486.1 alanine racemase -
GKS41_RS05095 1005635..1005916 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GKS41_RS05100 1005922..1006272 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GKS41_RS05105 1006390..1007211 + 822 WP_159353821.1 STAS domain-containing protein -
GKS41_RS05110 1007216..1007581 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
GKS41_RS05115 1007584..1007985 + 402 WP_003156178.1 anti-sigma regulatory factor -
GKS41_RS05120 1007997..1009004 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
GKS41_RS05125 1009068..1009397 + 330 WP_159353822.1 anti-sigma factor antagonist RsbV -
GKS41_RS05130 1009394..1009876 + 483 WP_007609591.1 anti-sigma B factor RsbW -
GKS41_RS05135 1009842..1010630 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
GKS41_RS05140 1010630..1011232 + 603 WP_128496463.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T145639 WP_003156187.1 NZ_CP047268:1005922-1006272 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T145639 NZ_CP062412:2670737-2671099 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT145639 WP_003156188.1 NZ_CP047268:1005635-1005916 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT145639 NZ_CP062412:2670570-2670740 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References