Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 5473960..5474571 Replicon chromosome
Accession NZ_CP047267
Organism Pseudomonas syringae UB303

Toxin (Protein)


Gene name PfiT Uniprot ID A0A3M3SLP6
Locus tag N026_RS24565 Protein ID WP_024659447.1
Coordinates 5473960..5474310 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID Q6VE98
Locus tag N026_RS24570 Protein ID WP_011152898.1
Coordinates 5474323..5474571 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N026_RS24550 5470083..5472848 + 2766 WP_201279924.1 RHS repeat protein -
N026_RS24555 5472845..5473252 + 408 WP_024659445.1 hypothetical protein -
N026_RS24560 5473450..5473854 + 405 WP_024659446.1 DUF4279 domain-containing protein -
N026_RS24565 5473960..5474310 - 351 WP_024659447.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N026_RS24570 5474323..5474571 - 249 WP_011152898.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
N026_RS24575 5474946..5475863 + 918 Protein_4661 tyrosine-type recombinase/integrase -
N026_RS24580 5475982..5476296 - 315 WP_024659449.1 hypothetical protein -
N026_RS24585 5476351..5477271 - 921 WP_024659450.1 hypothetical protein -
N026_RS24590 5477312..5477683 - 372 WP_145958166.1 hypothetical protein -
N026_RS24595 5477710..5477964 - 255 WP_024659451.1 hypothetical protein -
N026_RS24600 5478124..5478384 - 261 WP_024659452.1 hypothetical protein -
N026_RS24605 5478504..5478752 - 249 WP_024659453.1 hypothetical protein -
N026_RS24610 5478824..5479015 - 192 WP_050586783.1 antirestriction protein ArdR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5454550..5483942 29392


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12947.79 Da        Isoelectric Point: 4.1953

>T145638 WP_024659447.1 NZ_CP047267:c5474310-5473960 [Pseudomonas syringae UB303]
MNTFALRFTEVAQQSIEDQVEHLAITQGFSSAAQRIDTLLDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGITDIAVLLVLGGKQSVEQALIRYCLLQPM

Download         Length: 351 bp

>T145638 NZ_CP062412:2651149-2651358 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9329.71 Da        Isoelectric Point: 9.8452

>AT145638 WP_011152898.1 NZ_CP047267:c5474571-5474323 [Pseudomonas syringae UB303]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDEAIALVKLLAISSREYAQGKHCSTDELKARLSRRFA
HK

Download         Length: 249 bp

>AT145638 NZ_CP062412:2650844-2651137 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M3SLP6

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8LY57

References