Detailed information of TA system TA145634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 1710930..1711551
• Replicon: chromosome
• Accession: NZ_CP047265
• Organism: Pseudomonas asturiensis strain CC1524

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: N015_RS07580
• Protein ID: WP_024686216.1
• Coordinates: 1710930..1711235 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A7Z2QX60
• Locus tag: N015_RS07585
• Protein ID: WP_024686217.1
• Coordinates: 1711228..1711551 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N015_RS07555	1706705..1707412	-	708	WP_024686211.1	tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO	-
N015_RS07560	1707423..1707884	-	462	WP_024686212.1	DUF1456 family protein	-
N015_RS07565	1707952..1708662	-	711	WP_024686213.1	rRNA pseudouridine synthase	-
N015_RS07570	1708754..1709197	-	444	WP_024686214.1	GNAT family N-acetyltransferase	-
N015_RS07575	1709302..1710645	-	1344	WP_024686215.1	30S ribosomal protein S12 methylthiotransferase RimO	-
N015_RS07580	1710930..1711235	+	306	WP_024686216.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
N015_RS07585	1711228..1711551	+	324	WP_024686217.1	helix-turn-helix domain-containing protein	Antitoxin
N015_RS07590	1711764..1713659	+	1896	WP_024686218.1	potassium transporter Kup	-
N015_RS07595	1713738..1715081	-	1344	WP_024686219.1	virulence factor family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (1-88)

Antitoxin

• HigA2-like, helix-turn-helix domain (11-90)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11165.57 Da
• Isoelectric Point: 7.4179

>T145634 WP_024686216.1 NZ_CP047265:1710930-1711235 [Pseudomonas asturiensis]
MPEDVQDVFGYALHQAQQGNKHVQAVPLKGFSGAGVLEVVEDYDGDTYRAVYTVKFGSAVYALHCFQKKSTSGIKTAQHD
IDLIKSRLRAAEKHAKEVKND

Nucleotide

• Length: 306 bp

>T145634 NZ_CP062412:2280274-2280540 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATAATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11725.69 Da
• Isoelectric Point: 10.1465

>AT145634 WP_024686217.1 NZ_CP047265:1711228-1711551 [Pseudomonas asturiensis]
MTDIEESSGNVYADLGRDDAKEMLIKAQLATKIGAIIKARHLTQVEAARLLAMPQPKVSEMLRGKFRGISESKMIDCLSR
LGRDVQIVVKTAPRSRGGEGRVEVVFA

Nucleotide

• Length: 324 bp

>AT145634 NZ_CP062412:2280017-2280274 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7Z2QX60