Detailed information of TA system TA145632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 5686097..5686736
• Replicon: chromosome
• Accession: NZ_CP047260
• Organism: Pseudomonas syringae pv. maculicola str. ES4326

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: PMA4326_RS26155
• Protein ID: WP_007252702.1
• Coordinates: 5686554..5686736 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A8T8C7U3
• Locus tag: PMA4326_RS26150
• Protein ID: WP_054079873.1
• Coordinates: 5686097..5686522 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PMA4326_RS26135	5682568..5683890	+	1323	WP_054079875.1	murein hydrolase activator EnvC	-
PMA4326_RS26140	5683924..5685255	+	1332	WP_007252705.1	S41 family peptidase	-
PMA4326_RS26145	5685259..5686041	+	783	WP_054079874.1	divergent polysaccharide deacetylase family protein	-
PMA4326_RS26150	5686097..5686522	-	426	WP_054079873.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PMA4326_RS26155	5686554..5686736	-	183	WP_007252702.1	type II toxin-antitoxin system HicA family toxin	Toxin
PMA4326_RS26160	5687054..5688568	+	1515	WP_007252701.1	serine protease	-
PMA4326_RS26165	5688549..5689304	-	756	WP_007252700.1	ABC transporter substrate-binding protein	-
PMA4326_RS26170	5689492..5690262	-	771	WP_002551458.1	imidazole glycerol phosphate synthase subunit HisF	-
PMA4326_RS26175	5690282..5691019	-	738	WP_007252699.1	1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase	-
PMA4326_RS26180	5691125..5691385	-	261	WP_007252698.1	DUF2164 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-58)

Antitoxin

• DNA binding HTH domain, Psq-type (86-112)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6850.95 Da
• Isoelectric Point: 10.6211

>T145632 WP_007252702.1 NZ_CP047260:c5686736-5686554 [Pseudomonas syringae pv. maculicola str. ES4326]
MKCSEFRRWLQAQGAEFRAAKGSHFKVYLNGKATIFADHGSKEMHEGLRKSIIKQLGLKD

Nucleotide

• Length: 183 bp

>T145632 NZ_CP062412:1169554-1169742 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15787.27 Da
• Isoelectric Point: 5.2482

>AT145632 WP_054079873.1 NZ_CP047260:c5686522-5686097 [Pseudomonas syringae pv. maculicola str. ES4326]
MYQYPLDQHLEPTGVWLSCPDIPEMSASGDTLTEALSEALDGMESALSLYVEQRRKIPQASVVPGHELLMRLPALTVAKI
MLWNSLLEEGVSRAELARRLGCTRQVVYRLVDFLHASKIEQVERALAELGRRITLSIERAA

Nucleotide

• Length: 426 bp

>AT145632 NZ_CP062412:1169265-1169525 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA