Detailed information of TA system TA145624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-couple_hipB
• Location: 971654..972906
• Replicon: chromosome
• Accession: NZ_CP047248
• Organism: Streptococcus suis strain LSM178

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: A0A0Z9JWN4
• Locus tag: GRI05_RS04780
• Protein ID: WP_011922437.1
• Coordinates: 972130..972906 (+)
• Length: 259 a.a.

Antitoxin (Protein)

• Gene name: sezA
• Uniprot ID: -
• Locus tag: GRI05_RS04775
• Protein ID: WP_012775115.1
• Coordinates: 971654..972130 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRI05_RS04745	966824..967423	+	600	WP_011922444.1	response regulator transcription factor	-
GRI05_RS04750	968824..969462	+	639	WP_011922443.1	hypothetical protein	-
GRI05_RS04755	969501..970577	+	1077	WP_011922442.1	toprim domain-containing protein	-
GRI05_RS04760	970631..970858	+	228	WP_011922441.1	hypothetical protein	-
GRI05_RS04765	970855..971244	+	390	WP_011922440.1	methyl-accepting chemotaxis protein	-
GRI05_RS04770	971294..971584	+	291	WP_024379089.1	hypothetical protein	-
GRI05_RS04775	971654..972130	+	477	WP_012775115.1	helix-turn-helix domain-containing protein	Antitoxin
GRI05_RS04780	972130..972906	+	777	WP_011922437.1	zeta toxin family protein	Toxin
GRI05_RS04785	972903..974990	+	2088	WP_011922436.1	AAA family ATPase	-
GRI05_RS04790	974977..976794	+	1818	WP_012775114.1	ATP-dependent helicase	-
GRI05_RS04795	976889..977206	-	318	Protein_900	ATP-binding protein	-
GRI05_RS04800	977550..977864	+	315	WP_000420682.1	YdcP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(6)-Ia / erm(B) / tet(M)	-	921107..1022339	101232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 259 a.a.
• Molecular weight: 29171.98 Da
• Isoelectric Point: 5.2489

>T145624 WP_011922437.1 NZ_CP047248:972130-972906 [Streptococcus suis]
MRLEEFSEAAFQKALQRTIRALIRGKTIPEQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEHLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLASKGYQVSLAVIGTKPELSYLSTLIRYEE
LYAIDPNQARATPKEHHDGIVENLVDNLSELEREQLFDQIQIYQRDRTCIYDSEIDEGSAVEVLQECLFGQWSKVEEEML
KAGQERLGELVSANGEKK

Nucleotide

• Length: 777 bp

>T145624 NZ_CP062411:2353201-2353467 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18282.69 Da
• Isoelectric Point: 4.4750

>AT145624 WP_012775115.1 NZ_CP047248:971654-972130 [Streptococcus suis]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYHLTLKVE
VIKERGAAILSQLYRYQDSQDIAFDDESNPWILMSDDLAELINTKIYLVDTFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

>AT145624 NZ_CP062411:2352950-2353201 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A0Z9JWN4