Detailed information of TA system TA145611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1054625..1055213
• Replicon: chromosome
• Accession: NZ_CP047232
• Organism: Brucella sp. BO3

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7L7ML11
• Locus tag: GRI33_RS04980
• Protein ID: WP_008505629.1
• Coordinates: 1054625..1054915 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7L7ML36
• Locus tag: GRI33_RS04985
• Protein ID: WP_008505633.1
• Coordinates: 1054917..1055213 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRI33_RS04950	1050276..1051541	-	1266	WP_069715237.1	septal ring lytic transglycosylase RlpA family protein	-
GRI33_RS04955	1051913..1052260	-	348	WP_069715084.1	arsenate reductase (glutaredoxin)	-
GRI33_RS04960	1052332..1052436	-	105	Protein_976	lysozyme	-
GRI33_RS04965	1052504..1052740	-	237	Protein_977	hypothetical protein	-
GRI33_RS04970	1052962..1053714	-	753	WP_141710268.1	tail fiber protein	-
GRI33_RS04975	1053851..1054453	+	603	WP_141710267.1	hypothetical protein	-
GRI33_RS04980	1054625..1054915	+	291	WP_008505629.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GRI33_RS04985	1054917..1055213	+	297	WP_008505633.1	putative addiction module antidote protein	Antitoxin
GRI33_RS04990	1055302..1055568	+	267	WP_069715080.1	hypothetical protein	-
GRI33_RS04995	1055639..1056016	+	378	WP_069715079.1	hypothetical protein	-
GRI33_RS05000	1056113..1056505	+	393	WP_182231263.1	hypothetical protein	-
GRI33_RS05005	1056595..1056873	-	279	WP_069715077.1	hypothetical protein	-
GRI33_RS05010	1056986..1057750	-	765	WP_069715076.1	KilA-N domain-containing protein	-
GRI33_RS05015	1057764..1058006	-	243	WP_069715075.1	Arc family DNA-binding protein	-
GRI33_RS05020	1058003..1058170	-	168	WP_083262255.1	Arc family DNA-binding protein	-
GRI33_RS05025	1058170..1058934	-	765	WP_083262252.1	phage antirepressor KilAC domain-containing protein	-
GRI33_RS05030	1059244..1059633	+	390	WP_083262250.1	Arc family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1047170..1060705	13535
	-	flank	IS/Tn	-	-	1059749..1060705	956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10724.46 Da
• Isoelectric Point: 10.2779

>T145611 WP_008505629.1 NZ_CP047232:1054625-1054915 [Brucella sp. BO3]
MIEVRQTTAFTKWLNELRDTNARLRIATRIRRMELGNPGDVKAVGEGVSEMRITYGPGYRVYFVTMGSTIVVLLCGGDKS
SQSRDIAVAKQMAKEI

Nucleotide

• Length: 291 bp

>T145611 NZ_CP062410:1198733-1198921 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10440.88 Da
• Isoelectric Point: 5.1057

>AT145611 WP_008505633.1 NZ_CP047232:1054917-1055213 [Brucella sp. BO3]
MALETTRFDILDHLKTPEERLAYLEAAFEDGDPSLIAHALGDVARSVGMTAVAKEAGITREALYRSLSENGDPKLSTLLG
VTKALGIHLTVGPDKHAA

Nucleotide

• Length: 297 bp

>AT145611 NZ_CP062410:1198444-1198704 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A7L7ML11

Antitoxin

• AlphaFold DB: A0A7L7ML36