Detailed information of TA system TA145609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4413353..4414167
• Replicon: chromosome
• Accession: NZ_CP047231
• Organism: Escherichia coli strain BL21-AI

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: GSU80_RS21215
• Protein ID: WP_001054376.1
• Coordinates: 4413910..4414167 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: GSU80_RS21210
• Protein ID: WP_001309181.1
• Coordinates: 4413353..4413898 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSU80_RS21185	4409044..4410357	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
GSU80_RS21190	4410369..4410647	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
GSU80_RS21195	4410644..4411765	-	1122	WP_000010829.1	M42 family metallopeptidase	-
GSU80_RS21850	4412010..4412126	-	117	Protein_4121	VOC family protein	-
GSU80_RS21200	4412164..4412382	-	219	Protein_4122	hypothetical protein	-
GSU80_RS21205	4412551..4413297	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
GSU80_RS21210	4413353..4413898	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
GSU80_RS21215	4413910..4414167	-	258	WP_001054376.1	hypothetical protein	Toxin
GSU80_RS21220	4414544..4414789	-	246	Protein_4126	GNAT family N-acetyltransferase	-
GSU80_RS21225	4414905..4416145	+	1241	Protein_4127	DNA helicase	-
GSU80_RS21230	4416728..4417708	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
GSU80_RS21235	4417773..4418879	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	4371764..4423523	51759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T145609 WP_001054376.1 NZ_CP047231:c4414167-4413910 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T145609 NZ_CP062410:123401-123553 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT145609 WP_001309181.1 NZ_CP047231:c4413898-4413353 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT145609 NZ_CP062410:122777-123376 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8