Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2842887..2843541 Replicon chromosome
Accession NZ_CP047231
Organism Escherichia coli strain BL21-AI

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag GSU80_RS13730 Protein ID WP_000244783.1
Coordinates 2842887..2843294 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GSU80_RS13735 Protein ID WP_000354046.1
Coordinates 2843275..2843541 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSU80_RS13710 2838844..2840577 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
GSU80_RS13715 2840583..2841293 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GSU80_RS13720 2841318..2842214 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GSU80_RS13725 2842326..2842847 + 522 WP_001055874.1 flavodoxin FldB -
GSU80_RS13730 2842887..2843294 - 408 WP_000244783.1 protein YgfX Toxin
GSU80_RS13735 2843275..2843541 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GSU80_RS13740 2843784..2844764 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GSU80_RS13745 2844960..2845619 - 660 WP_000250274.1 hemolysin III family protein -
GSU80_RS13750 2845783..2846094 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
GSU80_RS13755 2846139..2847572 + 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
GSU80_RS13760 2847629..2848372 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T145605 WP_000244783.1 NZ_CP047231:c2843294-2842887 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T145605 NZ_CP062409:2640110-2640319 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT145605 WP_000354046.1 NZ_CP047231:c2843541-2843275 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT145605 NZ_CP062409:2639805-2640098 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References