Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2714956..2715539 Replicon chromosome
Accession NZ_CP047231
Organism Escherichia coli strain BL21-AI

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag GSU80_RS13190 Protein ID WP_000254738.1
Coordinates 2714956..2715291 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag GSU80_RS13195 Protein ID WP_000581937.1
Coordinates 2715291..2715539 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSU80_RS13175 2710843..2712141 - 1299 WP_000036723.1 phosphopyruvate hydratase -
GSU80_RS13180 2712229..2713866 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
GSU80_RS13185 2714094..2714885 - 792 WP_001071638.1 nucleoside triphosphate pyrophosphohydrolase -
GSU80_RS13190 2714956..2715291 - 336 WP_000254738.1 endoribonuclease MazF Toxin
GSU80_RS13195 2715291..2715539 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GSU80_RS13200 2715617..2717851 - 2235 WP_000226815.1 GTP pyrophosphokinase -
GSU80_RS13205 2717899..2719200 - 1302 WP_000046810.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T145604 WP_000254738.1 NZ_CP047231:c2715291-2714956 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T145604 NZ_CP062409:2582714-2582818 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT145604 WP_000581937.1 NZ_CP047231:c2715539-2715291 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT145604 NZ_CP062409:c2582976-2582813 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAATGCCGGTCAAAGCGAATAGAAGGTT
ATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References