Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 451582..452200 Replicon chromosome
Accession NZ_CP047231
Organism Escherichia coli strain BL21-AI

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GSU80_RS02115 Protein ID WP_001291435.1
Coordinates 451582..451800 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GSU80_RS02120 Protein ID WP_000344800.1
Coordinates 451826..452200 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSU80_RS02080 446871..447443 + 573 WP_000779826.1 lipoprotein -
GSU80_RS02085 447474..447785 - 312 WP_000409911.1 MGMT family protein -
GSU80_RS02095 448164..448517 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GSU80_RS02100 448559..450109 - 1551 WP_001319841.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GSU80_RS02105 450273..450743 - 471 WP_000136192.1 YlaC family protein -
GSU80_RS02110 450859..451410 - 552 WP_000102564.1 maltose O-acetyltransferase -
GSU80_RS02115 451582..451800 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GSU80_RS02120 451826..452200 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GSU80_RS02125 452746..455895 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GSU80_RS02130 455918..457111 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T145587 WP_001291435.1 NZ_CP047231:c451800-451582 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T145587 NZ_CP062408:18898-19074 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGATCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT145587 WP_000344800.1 NZ_CP047231:c452200-451826 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT145587 NZ_CP062408:c19236-19062 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References