Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 415921..416758 Replicon chromosome
Accession NZ_CP047231
Organism Escherichia coli strain BL21-AI

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag GSU80_RS01940 Protein ID WP_000227786.1
Coordinates 415921..416463 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag GSU80_RS01945 Protein ID WP_001297137.1
Coordinates 416447..416758 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSU80_RS01920 411451..412362 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
GSU80_RS01925 412530..413021 + 492 WP_001138904.1 nucleotide binding protein YajQ -
GSU80_RS01930 413149..414513 - 1365 WP_001000978.1 MFS transporter -
GSU80_RS01935 414930..415865 + 936 WP_001297127.1 sel1 repeat family protein -
GSU80_RS01940 415921..416463 - 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
GSU80_RS01945 416447..416758 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
GSU80_RS01950 416943..417833 - 891 WP_000971336.1 heme o synthase -
GSU80_RS01955 417845..418174 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
GSU80_RS01960 418174..418788 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
GSU80_RS01965 418778..420769 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
GSU80_RS01970 420791..421738 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T145586 WP_000227786.1 NZ_CP047231:c416463-415921 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T145586 NZ_CP062407:2801981-2802232 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCCGTTATCAGTGCTAGTGCGTATAACGGTAATGATACAGAGGGATTACTAAA
AGAGATTGAGGACGTATATAAGAAAGCGCGAGCGTTTGATGAAATACTTGAAGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT145586 WP_001297137.1 NZ_CP047231:c416758-416447 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT145586 NZ_CP062407:2802222-2802404 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTACAAGTTATCTGACGGCTCTATTATCAGTCTCATAGACTTTGTTATTAAACCAATTCATTTAATCA
AGGAGGCACAGGAAAATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References