Detailed information of TA system TA145577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 5879..6224
• Replicon: plasmid unnamed3
• Accession: NZ_CP047220
• Organism: Sphingobium yanoikuyae strain YC-JY1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: GS397_RS26745
• Protein ID: WP_159368255.1
• Coordinates: 5879..6007 (+)
• Length: 43 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: GS397_RS26750
• Protein ID: WP_159368256.1
• Coordinates: 5994..6224 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS397_RS26710	3249..3494	+	246	WP_201450357.1	ribbon-helix-helix domain-containing protein	-
GS397_RS26715	3498..3932	+	435	WP_159368251.1	putative toxin-antitoxin system toxin component, PIN family	-
GS397_RS26720	3929..4648	+	720	WP_159368252.1	hypothetical protein	-
GS397_RS26725	4677..4934	-	258	Protein_4	hypothetical protein	-
GS397_RS26730	4977..5159	-	183	Protein_5	cytoplasmic protein	-
GS397_RS26735	5182..5475	-	294	WP_159368253.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GS397_RS26740	5468..5746	-	279	WP_159368254.1	type II toxin-antitoxin system ParD family antitoxin	-
GS397_RS26745	5879..6007	+	129	WP_159368255.1	hypothetical protein	Toxin
GS397_RS26750	5994..6224	+	231	WP_159368256.1	BrnA antitoxin family protein	Antitoxin
GS397_RS26755	6256..6583	+	328	Protein_10	hypothetical protein	-
GS397_RS26760	6626..7018	-	393	Protein_11	IS66 family insertion sequence element accessory protein TnpB	-
GS397_RS26765	6920..8293	+	1374	WP_159368257.1	hypothetical protein	-
GS397_RS26770	8730..9011	+	282	WP_082635049.1	WGR domain-containing protein	-
GS397_RS26775	9026..9121	-	96	Protein_14	histidine phosphatase family protein	-
GS397_RS26780	9139..9432	-	294	WP_159368258.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GS397_RS26785	9420..9698	-	279	WP_159368259.1	CopG family ribbon-helix-helix protein	-
GS397_RS26790	10078..10638	+	561	WP_159368303.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..65025	65025
	-	flank	IS/Tn	-	-	10078..10638	560

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (11-69)

Sequences

Toxin

Protein

• Length: 43 a.a.
• Molecular weight: 4755.39 Da
• Isoelectric Point: 6.7185

>T145577 WP_159368255.1 NZ_CP047220:5879-6007 [Sphingobium yanoikuyae]
MGLIGDRLHVLVFTEGSHDDAVRAISLRPAEKHEARFYYGQV

Nucleotide

• Length: 129 bp

>T145577 NZ_CP062407:1825768-1826034 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATGATCCTTGTAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8321.61 Da
• Isoelectric Point: 10.4807

>AT145577 WP_159368256.1 NZ_CP047220:5994-6224 [Sphingobium yanoikuyae]
MAKFDPEIHDDNPPMDAAFMAGMKPSRRGRPKSEAPKVEVKIRLDAKTVEHLRGSGPGWQTRVNALLGQLVATGQL

Nucleotide

• Length: 231 bp

>AT145577 NZ_CP062407:1825511-1825768 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAGCATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTCAGAGAAAGAGAAAAAGATAATAGTGGTACTACAAATATAGACGATATT
GATTGGGATAATCTTTAA